Which Lymphoid Cells Express MHC Class II Antigens; Are TCRs Encoded within the MHC?

Which lymphoid cells express MHC class II antigens; are TCRs encoded within the MHC?

MHC class II tetramers.

MHC class II tetramers have emerged as an important tool for characterization of the specificity and phenotype of CD4 T cell immune responses, useful in a large variety of disease and vaccine studies. Issues of specific T cell frequency, biodistribution, and avidity, coupled with the large genetic diversity of potential class II restriction elements, require targeted experimental design. Transl...

Expression of MHC Class II Antigens During Xenopus Development

Larval and adult forms of the amphibian Xenopus differ in their MHC class II expression. In tadpoles, class II epitopes can be detected by monoclonal antibodies only on B cells, macrophages (whatever their location), spleen reticulum, thymus epithelium, and the pharyngobuccal cavity. In contrast, all adult T cells express class II on their surface. The transitions in class II expression occur a...

Tumor cells present MHC class II-restricted nuclear and mitochondrial antigens and are the predominant antigen presenting cells in vivo.

MHC class II-restricted tumor Ags presented by class II(+) tumor cells identified to date are derived from proteins expressed in the cytoplasm or plasma membrane of tumor cells. It is unclear whether MHC class II(+) tumor cells present class II-restricted epitopes derived from other intracellular compartments, such as nuclei and/or mitochondria, and whether class II(+) tumor cells directly pres...

Dynamics within tetraspanin pairs affect MHC class II expression.

Late endosomal multivesicular bodies (MVBs) are complicated organelles with various subdomains located at the limiting membrane and the internal vesicles (ILVs). ILVs accumulate tetraspanins such as CD63 and CD82 that might form protein assemblies, including major histocompatibility complex class II (MHC-II) and its chaperone human leukocyte antigen (HLA)-DM. Here, we studied the effect of four...